000			02714 am a22003493u 4500
042			_adc
100	1	0	_aShkoporov, Andrey N. _eauthor _92042
700	1	0	_aStockdale, Stephen R. _eauthor _92043
700	1	0	_aLavelle, Aonghus _eauthor _92044
700	1	0	_aKondova, Ivanela _eauthor _92045
700	1	0	_aHeuston, Cara _eauthor _92046
700	1	0	_aUpadrasta, Aditya _eauthor _92047
700	1	0	_aKhokhlova, Ekaterina V. _eauthor _92048
700	1	0	_avan der Kamp, Imme _eauthor _92049
700	1	0	_aOuwerling, Boudewijn _eauthor _92050
700	1	0	_aDraper, Lorraine A. _eauthor _92051
700	1	0	_aLangermans, Jan A.M. _eauthor _92052
700	1	0	_aRoss, R Paul _eauthor _92053
700	1	0	_aHill, Colin _eauthor _92054
245	0	0	_aViral biogeography of the mammalian gut and parenchymal organs
260			_c2022-08-01.
500			_a/pmc/articles/PMC7614033/
500			_a/pubmed/35918425
520			_aThe mammalian virome has been linked to health and disease but our understanding of how it is structured along the longitudinal axis of the mammalian gastrointestinal tract (GIT) and other organs is limited. Here we report a metagenomic analysis of the prokaryotic and eukaryotic virome occupying luminal and mucosa-associated habitats along the GIT, as well as parenchymal organs (liver, lung and spleen), in two representative mammalian species, the domestic pig and rhesus macaque (six animals per species). Luminal samples from the large intestine of both mammals harboured the highest loads and diversity of bacteriophages (class Caudoviricetes, family Microviridae and others). Mucosal samples contained much lower viral loads but a higher proportion of eukaryotic viruses (families Astroviridae, Caliciviridae, Parvoviridae). Parenchymal organs contained significant numbers of bacteriophages of gut origin, in addition to some eukaryotic viruses. Overall, GIT virome composition was specific to anatomical region and host species. Upper GIT and mucosa-specific viruses were greatly under-represented in distal colon samples (a proxy for faeces). Nonetheless, certain viral and phage species were found ubiquitously in all samples from the oral cavity to the distal colon. The dataset and its accompanying methodology may provide an important resource for future work investigating the biogeography of the mammalian gut virome.
540			_a
540			_ahttps://creativecommons.org/licenses/by/4.0/This work is licensed under a CC BY 4.0 (https://creativecommons.org/licenses/by/4.0/) International license.
546			_aen
690			_aArticle
655	7		_aText _2local
786	0		_nNat Microbiol
856	4	1	_uhttp://dx.doi.org/10.1038/s41564-022-01178-w _zConnect to this object online.
999			_c869 _d869