| 000 | 02423 am a22003013u 4500 | ||
|---|---|---|---|
| 042 | _adc | ||
| 100 | 1 | 0 |
_aMartinez, Matthew _eauthor _91245 |
| 700 | 1 | 0 |
_aChen, William David _eauthor _91246 |
| 700 | 1 | 0 |
_aCova, Marta Mendonça _eauthor _91247 |
| 700 | 1 | 0 |
_aMolnár, Petra _eauthor _91248 |
| 700 | 1 | 0 |
_aMageswaran, Shrawan Kumar _eauthor _91249 |
| 700 | 1 | 0 |
_aGuérin, Amandine _eauthor _91250 |
| 700 | 1 | 0 |
_aJohn, Audrey R. Odom _eauthor _91251 |
| 700 | 1 | 0 |
_aLebrun, Maryse _eauthor _91252 |
| 700 | 1 | 0 |
_aChang, Yi-Wei _eauthor _91253 |
| 245 | 0 | 0 | _aRhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography |
| 260 | _c2022-07-11. | ||
| 500 | _a/pmc/articles/PMC7613239/ | ||
| 500 | _a/pubmed/35817892 | ||
| 520 | _aApicomplexan parasites secrete contents of the rhoptries, club-shaped organelles in the apical region, into host cells to permit their invasion and establishment of infection. The rhoptry secretory apparatus (RSA), which is critical for rhoptry secretion, was recently discovered in Toxoplasma and Cryptosporidium. It is unknown if a similar molecular machinery exists in the malaria parasite Plasmodium. In this study, we use in situ cryo-electron tomography to investigate the rhoptry secretion system in P. falciparum merozoites. We identify the presence of an RSA at the cell apex and a morphologically distinct apical vesicle docking the tips of the two rhoptries to the RSA. We also discover two additional rhoptry organizations that lack the apical vesicle. Using subtomogram averaging, we reveal different conformations of the RSA structure corresponding to different rhoptry organizations. Our results highlight previously unknown steps in the process of rhoptry secretion and indicate a regulatory role for the conserved apical vesicle in host invasion by apicomplexan parasites. | ||
| 540 | _a | ||
| 540 | _ahttps://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms | ||
| 546 | _aen | ||
| 690 | _aArticle | ||
| 655 | 7 |
_aText _2local |
|
| 786 | 0 | _nNat Microbiol | |
| 856 | 4 | 1 |
_uhttp://dx.doi.org/10.1038/s41564-022-01171-3 _zConnect to this object online. |
| 999 |
_c784 _d784 |
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