| 000 | 02788 am a22003133u 4500 | ||
|---|---|---|---|
| 042 | _adc | ||
| 100 | 1 | 0 |
_aSefkow-Werner, Julius _eauthor _92301 |
| 700 | 1 | 0 |
_aLe Pennec, Jean _eauthor _92302 |
| 700 | 1 | 0 |
_aMachillot, Paul _eauthor _92303 |
| 700 | 1 | 0 |
_aNdayishimiye, Bertin _eauthor _92304 |
| 700 | 1 | 0 |
_aCastro-Ramirez, Elaine _eauthor _92305 |
| 700 | 1 | 0 |
_aLopes, Joao _eauthor _92306 |
| 700 | 1 | 0 |
_aLicitra, Christophe _eauthor _92307 |
| 700 | 1 | 0 |
_aWang, Irene _eauthor _92308 |
| 700 | 1 | 0 |
_aDelon, Antoine _eauthor _92309 |
| 700 | 1 | 0 |
_aPicart, Catherine _eauthor |
| 700 | 1 | 0 |
_aMigliorini, Elisa _eauthor |
| 245 | 0 | 0 | _aAutomated fabrication of streptavidin-based self-assembled materials for high-content analysis of cellular response to growth factors |
| 260 | _c2022-07-18. | ||
| 500 | _a/pmc/articles/PMC7614070/ | ||
| 500 | _a/pubmed/35849638 | ||
| 520 | _aThe automation of liquid handling routines offers great potential for fast, reproducible and labor-reduced biomaterials fabrication but also requires the development of special protocols. Competitive systems demand for a high degree in miniaturization and parallelization while maintaining flexibility regarding the experimental design. To day, there are only few possibilities for automated fabrication of biomaterials inside multi-well plates. We have previously demonstrated that streptavidin-based biomimetic platforms can be employed to study cellular behaviors on biomimetic surfaces. So far, these self-assembled materials were made by stepwise assembly of the components using manual pipetting. In this work, we introduce for the first time a fully automated and adaptable workflow to functionalize glass-bottom multi-well plates with customized biomimetic platforms deposited in single wells using a liquid handling robot. We then characterize cell response using automated image acquisition and subsequent analysis. Furthermore, the molecular surface density of the biomimetic platforms was characterized in situ using fluorescence-based image correlation spectroscopy. These measurements were in agreement with standard ex situ spectroscopic ellipsometry measurements. Thanks to automation, we could do a proof-of-concept to study the effect of heparan sulfate on the bioactivity of bone morphogenetic proteins on myoblast cells, using four different BMPs (2,4,6 and 7) in parallel, at five increasing concentrations. Using such automated self-assembly of biomimetic materials, it may be envisioned to further investigate the role of a large variety of ECM components and growth factors on cell signaling. | ||
| 540 | _a | ||
| 546 | _aen | ||
| 690 | _aArticle | ||
| 655 | 7 |
_aText _2local |
|
| 786 | 0 | _nACS Appl Mater Interfaces | |
| 856 | 4 | 1 |
_uhttp://dx.doi.org/10.1021/acsami.2c08272 _zConnect to this object online. |
| 999 |
_c2235 _d2235 |
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