JOM KITA KE POLITEKNIK

Expression and Characterization of a Family 45 Glycosylhydrolase from Fomitopsis pinicola and comparison to Phanerochaete chrysosporium Cel45A (Record no. 1939)

MARC details
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100 10 - MAIN ENTRY--PERSONAL NAME
Personal name Amengual, Neus Gacias
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9 (RLIN) 1428
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Title Expression and Characterization of a Family 45 Glycosylhydrolase from Fomitopsis pinicola and comparison to Phanerochaete chrysosporium Cel45A
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Date of publication, distribution, etc. 2022-05-01.
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General note /pmc/articles/PMC7613719/
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General note /pubmed/35123123
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Summary, etc. To efficiently decompose biomass, fungi have developed various enzymatic and non-enzymatic strategies and are a source of versatile biocatalysts. The endoglucanases in glycosyl hydrolase CAZy family 45 (GH45) are known for their small size, a high thermostability and a broad substrate specificity that has been employed in textile and detergent industries. Here we report the heterologous expression and characterisation of an GH45 endoglucanase from the brown rot Fomitopsis pinicola and its direct comparison to an already characterised GH45 from the white rot Phanerochaete chrysosporium. Both enzymes were recombinantly expressed in Pichia pastoris and purified by two chromatographic steps. The biochemical characterisation highlighted the acidophilic character, with an optimal pH of 4, and a preference for amorphous substrates as carboxymethyl cellulose (CMC) and substrates containing β-1,4-glucans rather than the previously reported β-1,3/1,4-glucans lichenan and β-glucan. The dominating products from β-1,4-glucans were C3-C6 oligosaccharides, whereas from β-1,3/1,4-glucans glucose was the main reaction product. From the characterisation no differences between the brown rot and the white rot GH45 was evident.
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Language note en
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Topical term or geographic name as entry element Article
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700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Csarman, Florian
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700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Wohlschlager, Lena
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9 (RLIN) 1430
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Ludwig, Roland
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Note Enzyme Microb Technol
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Uniform Resource Identifier <a href="http://dx.doi.org/10.1016/j.enzmictec.2022.110000">http://dx.doi.org/10.1016/j.enzmictec.2022.110000</a>
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