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Event-driven acquisition for content-enriched microscopy (Record no. 1935)

MARC details
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Personal name Mahecic, Dora
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9 (RLIN) 1419
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Title Event-driven acquisition for content-enriched microscopy
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Date of publication, distribution, etc. 2022-09-08.
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General note /pmc/articles/PMC7613693/
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General note /pubmed/36076039
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Summary, etc. A common goal of fluorescence microscopy is to collect data on specific biological events. Yet, the event-specific content that can be collected from a sample is limited, especially for rare or stochastic processes. This is due in part to photobleaching and phototoxicity, which constrain imaging speed and duration. We developed an event-driven acquisition (EDA) framework, in which neural network-based recognition of specific biological events triggers real-time control in an instant structured illumination microscope (iSIM). Our setup adapts acquisitions on-the-fly by switching between a slow imaging rate while detecting the onset of events, and a fast imaging rate during their progression. Thus, we capture mitochondrial and bacterial divisions at imaging rates that match their dynamic timescales, while extending overall imaging durations. Because EDA allows the microscope to respond specifically to complex biological events, it acquires data enriched in relevant content.
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Terms governing use and reproduction
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Terms governing use and reproduction https://www.springernature.com/gp/open-research/policies/accepted-manuscript-termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: https://www.springernature.com/gp/open-research/policies/accepted-manuscript-terms
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Language note en
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Topical term or geographic name as entry element Article
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Genre/form data or focus term Text
Source of term local
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Personal name Stepp, Willi L.
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9 (RLIN) 1420
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Personal name Zhang, Chen
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9 (RLIN) 1421
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Personal name Griffié, Juliette
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9 (RLIN) 1422
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Personal name Weigert, Martin
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9 (RLIN) 1423
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Personal name Manley, Suliana
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9 (RLIN) 1424
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Note Nat Methods
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Uniform Resource Identifier <a href="http://dx.doi.org/10.1038/s41592-022-01589-x">http://dx.doi.org/10.1038/s41592-022-01589-x</a>
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